https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:48589 Wed 22 Mar 2023 08:32:47 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:34453 Saccharomyces cerevisiae, the NuA3 histone acetyltransferase complex contains three domains, the PHD finger in Yng1, the PWWP domain in Pdp3, and the YEATS domain in Taf14; which in vitro bind to H3K4 methylation, H3K36 methylation, and acetylated and crotonylated H3K9, respectively. While the in vitro binding has been well characterized, the relative in vivo contributions of these histone PTMs in targeting NuA3 is unknown. Here, through genome-wide colocalization and by mutational interrogation, we demonstrate that the PHD finger of Yng1, and the PWWP domain of Pdp3 independently target NuA3 to H3K4 and H3K36 methylated chromatin, respectively. In contrast, we find no evidence to support the YEATS domain of Taf14 functioning in NuA3 recruitment. Collectively our results suggest that the presence of multiple histone PTM binding domains within NuA3, rather than restricting it to nucleosomes containing distinct combinations of histone PTMs, can serve to increase the range of nucleosomes bound by the complex. Interestingly, however, the simple presence of NuA3 is insufficient to ensure acetylation of the associated nucleosomes, suggesting a secondary level of acetylation regulation that does not involve control of HAT-nucleosome interactions.]]> Wed 13 Mar 2019 13:12:22 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:13894 Wed 11 Apr 2018 09:40:23 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:10028 Wed 11 Apr 2018 09:21:02 AEST ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:29462 IRAKM, PCDH1, ORMDL3/GSDMB, IL-33, CDHR3 and CST1 in airway epithelial cells. Recent studies on epigenetic regulatory factors have further provided novel insights to the field, particularly their effect on regulation of some of the asthma susceptibility genes (e.g. methylation of ADAM33). Among the epigenetic regulatory mechanisms, microRNA networks have been shown to regulate a major portion of post-transcriptional gene regulation. Particularly, miR-19a may have some therapeutic potential.]]> Thu 24 Mar 2022 11:32:30 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21041 Sat 24 Mar 2018 07:50:36 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:38008 Fri 23 Jul 2021 15:13:02 AEST ]]>